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Test Code CHIKS Chikungunya Virus, PCR, Molecular Detection, Serum


Additional Testing Requirements


Due to the short period in which chikungunya RNA may be detected in serum, testing serum for IgM- and IgG-class antibodies to chikungunya virus is also recommended. See CHIKV / Chikungunya IgM and IgG, Antibody, Serum.

 

Testing for RNA or IgM-class antibodies to dengue and Zika viruses, concurrently with chikungunya virus testing should also be considered, given the overlapping clinical syndromes seen with these viruses.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions:

1. Within 2 hours of collection, centrifuge and aliquot the serum into a sterile container.

2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.


Useful For

Qualitative detection of chikungunya virus in serum after early symptom onset (ideally <7 days)

 

This test is not recommended for screening healthy patients.

Method Name

Real-Time Reverse Transcription Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Chikungunya Virus, PCR, Serum

Specimen Type

Serum

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Gross hemolysis Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Reference values apply to all ages.

Interpretation

A positive test result indicates the presence of chikungunya virus RNA in the specimen.

 

A negative test result with a positive internal control indicates that chikungunya virus RNA is not detectable in the specimen.

 

A negative test result with a negative internal control is considered evidence of polymerase chain reaction inhibition or reagent failure. A new specimen should be collected for testing if clinically indicated.

Day(s) Performed

Tuesday, Thursday

Report Available

Same day/1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CHIKS Chikungunya Virus, PCR, Serum 81152-1

 

Result ID Test Result Name Result LOINC Value
603833 Chikungunya Virus, PCR, Serum 81152-1

Testing Algorithm

For more information see Mosquito-borne Disease Laboratory Testing.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.