Test Code CLLMF Chronic Lymphocytic Leukemia, Specified FISH, Varies
Ordering Guidance
This test is intended for instances when limited chronic lymphocytic leukemia (CLL) fluorescence in situ hybridization (FISH) probes are needed based on specific abnormalities or abnormalities identified in the diagnostic sample.
If targeted FISH probes are not included with this test order, test processing will be delayed and the test may be canceled and automatically reordered by the laboratory as CLLDF / Chronic Lymphocytic Leukemia, Diagnostic FISH, Varies.
If a paraffin-embedded tissue sample is received, this test will be canceled and automatically reordered by the laboratory as SLL / Small Lymphocytic Lymphoma, FISH, Tissue.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
A reason for testing and a flow cytometry and/or a bone marrow pathology report should be submitted with each specimen. The laboratory will not reject testing if this information is not provided; however, appropriate testing and/or interpretation may be compromised or delayed in some instances. If not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood in original tube. Do not aliquot.
Acceptable:
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 2 to 3 mL
Collection Instructions:
1. It is preferable to send the first aspirate from the bone marrow collection.
2. Invert several times to mix bone marrow.
3. Send bone marrow in original tube. Do not aliquot.
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Useful For
Detecting recurrent common chromosome abnormalities in patients with chronic lymphocytic leukemia (CLL)
Distinguishing patients with 11;14 translocations who have the leukemic phase of mantle cell lymphoma from patients who have CLL
Detecting patients with atypical CLL with translocations between IGH and BCL3
Evaluating specimens in which chromosome studies are unsuccessful
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
CLLMB | Probe, Each Additional (CLLMF) | No, (Bill Only) | No |
Testing Algorithm
This test includes a charge for the probe application, analysis, and professional interpretation of results for one probe set (2 individual fluorescence in situ hybridization [FISH] probes). Additional charges will be incurred for additional probe sets performed.
This is not intended as a panel test and the desired probes must be specified upon order. If the patient is being evaluated for known abnormalities, targeted probes must be listed in the probe request field.
When specified, any of the following probes will be performed:
6q-, D6Z1/MYB
11q-, D11Z1/ATM
+12, D12Z3/MDM2
13q-, D13S319/LAMP1
17p-, TP53/D17Z1
t(11;14), CCND1/IGH
t(14;19), IGH/BCL3
Appropriate ancillary probes may be performed at consultant discretion to render comprehensive assessment. Any additional probes will have the results included within the final report and will be performed at an additional charge.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
CLL, Specified FISHSpecimen Type
VariesSpecimen Minimum Volume
Blood: 2 mL; Bone marrow: 1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Ambient (preferred) | ||
Refrigerated |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
An interpretive report will be provided.
Interpretation
A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe set.
The absence of an abnormal clone does not rule out the presence of a chronic lymphocytic leukemia clone or another neoplastic disorder.
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
88271x2, 88275, 88291-FISH Probe, Analysis, Interpretation; 1 probe set
88271x2, 88275-FISH Probe, Analysis; each additional probe set (if appropriate)
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
CLLMF | CLL, Specified FISH | 101920-7 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
610725 | Result Summary | 50397-9 |
610726 | Interpretation | 69965-2 |
610727 | Result Table | 93356-4 |
610728 | Result | 62356-1 |
GC091 | Reason for Referral | 42349-1 |
GC093 | Probes Requested | 78040-3 |
GC092 | Specimen | 31208-2 |
610729 | Source | 31208-2 |
610730 | Method | 85069-3 |
610731 | Additional Information | 48767-8 |
610732 | Disclaimer | 62364-5 |
610733 | Released by | 18771-6 |