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HelpTest Code DHRF Dihydrorhodamine Flow Cytometric N-Formyl-Methionyl-Leucyl-Phenylalanine Test, Blood
Useful For
Evaluation of RAC2 deficiency and RAC2 gain of function
Reporting Name
DHR Flow fMLP, BSpecimen Type
WB Sodium HeparinShipping Instructions
Testing performed Monday through Friday. Specimens not received by 4 p.m. (CST) on Friday may be cancelled.
Collect and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the mailer. It is recommended that specimens arrive within 24 hours of collection.
Specimens arriving on the weekend and observed holidays may be canceled.
Necessary Information
Ordering healthcare professional name and phone number are required.
Specimen Required
Two whole blood sodium heparin specimens are required, one from the testing patient and the other from an unrelated healthy donor as a control.
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Patient:
Container/Tube: Green top (sodium heparin)
Specimen Volume: 5 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Normal Control:
Container/Tube: Green top (sodium heparin)
Specimen Volume: 5 mL
Collection Instructions:
1. Collect a control specimen from the unrelated healthy donor within an hour of the patient's specimen collection time.
2. Label clearly with Normal Control and the corresponding patient information.
3. Send the whole blood specimen in the original tube. Do not aliquot.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| WB Sodium Heparin | Ambient | 48 hours | GREEN TOP/HEP |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Reference Values
|
Result name |
Unit |
Cutoff for defining normal |
|
% fMLP ox-DHR+ |
% |
≥10% |
|
MFI fMLP ox-DHR+ |
MFI |
≥2 |
|
Control % fMLP ox-DHR+ |
% |
≥10% |
|
Control MFI fMLP ox-DHR+ |
MFI |
≥2 |
fMLP = N-formyl-methionyl-leucyl-phenylalanine
DHR = dihydrorhodamine
MFI = mean fluorescence intensity
The appropriate age-related reference values for Absolute Neutrophil Count will be provided on the report.
Interpretation
An interpretive report will be provided, in addition to the quantitative values.
Interpretation of the results of the quantitative dihydrorhodamine (DHR) flow cytometric assay has to include both the proportion of positive neutrophils for DHR after N-formyl-methionyl-leucyl-phenylalanine stimulation, and the mean fluorescence intensity.
Report Available
3 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86352
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DHRF | DHR Flow fMLP, B | 98123-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ANC | Absolute Neutrophil Count | 751-8 |
| FMPPP | % FMLP ox-DHR+ | 85373-9 |
| FMPM | MFI fMLP ox-DHR+ | 85370-5 |
| ANCC | Control Absolute Neutrophil Count | 85369-7 |
| FMPPC | Control % fMLP ox-DHR+ | 85372-1 |
| FMPMC | Control MFI fMLP ox-DHR+ | 85371-3 |
| DHRFI | Interpretation | 69052-9 |
Method Name
Flow Cytometry
Day(s) Performed
Monday through Friday