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HelpTest Code EGFRW EGFR Targeted Mutation Analysis with ALK Reflex, Tumor
Specimen Required
Pathology report must accompany specimen for testing to be performed.
Preferred:
Specimen Type: Tissue
Container/Tube: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block.
Acceptable:
Specimen Type: Tissue
Container/Tube: Slides
Specimen Volume: 12 unstained, positively charged, unbaked slides or 2 hematoxylin and eosin-stained slides (will not be returned) and 10 unstained, positively charged, unbaked slides
Collection Instructions: Submit 12 unstained, positively charged, unbaked slides cut at 5-microns or 2 hematoxylin and eosin-stained slides and 10 unstained, positively charged, unbaked slides with 5-micron thick sections of the tumor tissue.
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
Molecular Genetics: Inherited Cancer Syndromes Patient Information (T519)
Oncology Test Request (T729)
Useful For
Identifying non-small cell lung cancers that may benefit from treatment with epidermal growth factor receptor -targeted therapies or anaplastic lymphoma kinase inhibitors
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SLIRV | Slide Review in MG | No, (Bill Only) | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| LCAF | ALK (2p23), Lung Cancer, FISH, Ts | Yes | No |
Testing Algorithm
When this test is ordered, the EGFR Gene, Mutation Analysis, 51 Mutation Panel, Tumor will always be performed. All specimens without an EGFR mutation will be automatically reflexed to testing for the ALK (2p23) rearrangement. Specimens with an identified EGFR mutation will result in cancellation of the LCAF test.
When this test is ordered, slide review will always be performed at an additional charge.
Method Name
Polymerase Chain Reaction (PCR)
Reporting Name
EGFR with ALK Reflex, TumorSpecimen Type
VariesSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Specimens that have been decalcified (all methods) Low tumor percentage Insufficient amount of tumor Specimens that have not been formalin-fixed, paraffin-embedded |
Reject |
Reference Values
An interpretive report will be provided.
Interpretation
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
5 to 14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81235-EGFR (epidermal growth factor receptor) (eg, non-small cell lung cancer) gene analysis, common variants(eg, exon 19 deletions, L858R, T790M, G719S, L861Q)
88381-Microdissection, manual
88271 x 2-DNA Probe (if appropriate)
88274-Interphase in situ hybridization (if appropriate)
88291-Interpretation and report (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EGFRW | EGFR with ALK Reflex, Tumor | 21665-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 616130 | Result Summary | 50397-9 |
| 616131 | Result | 21665-5 |
| 616132 | Interpretation | 69047-9 |
| 616133 | Specimen | 31208-2 |
| 616134 | Source | 31208-2 |
| 616135 | Tissue ID | 80398-1 |
| 616136 | Released By | 18771-6 |