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Test Code HK1 Hexokinase Enzyme Activity, Blood


Specimen Required


Container/Tube:

Preferred: Yellow top (ACD solution B)

Acceptable: Lavender top (EDTA)

Specimen Volume: 6 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Useful For

The evaluation of individuals with Coombs-negative chronic hemolysis

Method Name

Kinetic Spectrophotometry (KS)

Reporting Name

Hexokinase, B

Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD-B Refrigerated 20 days

Reject Due To

Gross hemolysis Reject

Reference Values

≥ 12 months: 0.7-1.7 U/g Hb

Reference values have not been established for patients who are younger than12 months.

Interpretation

Clinical correlation or genetic confirmation may be required to establish hexokinase (HK) deficiency as a cause of hemolytic anemia as the assayed activity level in confirmed cases can vary from markedly decreased to borderline normal levels due to a compensated increase in enzyme by reticulocytes. Comparison of HK activity levels to other RBC enzyme activity can be very useful. Heterozygotes have moderately decreased to low normal HK levels and are expected to be clinically unaffected. 

 

Increased HK activity may be seen when reticulocytes are increased and is not supportive of a diagnosis of HK deficiency.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82657

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HK1 Hexokinase, B 49216-5

 

Result ID Test Result Name Result LOINC Value
HKCL Hexokinase, B 49216-5

Day(s) Performed

Tuesday, Thursday

Report Available

1 to 6 days

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.