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Test Code HV2QU HIV-2 RNA Detection and Quantification, Real-Time qRT-PCR, Plasma


Ordering Guidance


This test is indicated for either 1) confirmatory qualitative detection of HIV-2 RNA in individuals with indeterminate or positive HIV-2-specific antibody but negative HIV-1-specific antibody test results; or 2) baseline or serial monitoring of HIV-2 viral load in those with confirmed HIV-2 infection with or without antiretroviral therapy.



Shipping Instructions


Ship specimen frozen on dry ice. If shipment will be delayed for more than 24 hours, freeze specimen at -20 to -80° C (up to 35 days) before shipment and then transport on dry ice.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 2.1 mL Plasma

Collection Instructions: Centrifuge and aliquot plasma into a plastic vial.


Useful For

Confirmatory detection of human immunodeficiency virus type 2 (HIV-2) RNA in clinical plasma specimens for the diagnosis of acute or established HIV-2 infection

 

Quantifying plasma HIV-2 RNA levels (viral load) in individuals living with HIV-2:

-Before initiating antiretroviral therapy to obtain baseline viral load

-For monitoring HIV-2 disease progression with or without antiretroviral therapy

Method Name

Reverse Transcription Real-time Polymerase Chain Reaction (RT-qPCR)

Reporting Name

HIV-2 RNA Detect / Quant, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

Plasma: 1.1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 60 days ALIQUOT TUBE
  Refrigerated  6 days ALIQUOT TUBE

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

Undetected

Interpretation

The quantification range of this assay is 50 to 3,160,000 IU/mL (1.70 log to 6.50 log IU/mL), with a limit of detection (based on a 95% detection rate) of 18 IU/mL.

 

An "Undetected" result indicates that HIV-2 RNA is not detected in the plasma specimen (see Cautions). The limit of detection for this assay is 18 IU/mL.

 

A result of "<50 IU/mL" indicates that the HIV-2 RNA level present in the plasma specimen is below 50 IU/mL (1.70 log IU/mL), and the assay cannot accurately quantify the HIV-2 RNA present below this level.

 

A quantitative value (reported in IU/mL and log10 IU/mL) indicates the HIV-2 RNA level (i.e., viral load) present in the plasma specimen.

 

A result of ">3,160,000 IU/mL" indicates that the HIV-2 RNA level present in the plasma specimen is above 3,160,000 IU/mL (6.50 log IU/mL), and this assay cannot accurately quantify the HIV-2 RNA present above this level.

 

An "Indeterminate" result suggests the presence of an atypical HIV-2 target sequence. Since the HIV-2 RNA sequence is atypical, repeat testing is unlikely to change this result and therefore is not recommended.

 

An "Equivocal" result indicates that the presence or absence of HIV-2 RNA in the plasma specimen could not be determined with certainty due to atypical real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) probe reactivity. Submission of a new specimen for testing is recommended.

 

An "Inconclusive" result indicates that the presence or absence of HIV-2 RNA in the plasma specimen could not be determined with certainty after repeat testing in the laboratory, possibly due to RT-qPCR inhibition. Submission of a new specimen for testing is recommended.

Day(s) Performed

Varies (once per week)

Report Available

1 to 14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87539

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HV2QU HIV-2 RNA Detect / Quant, P 69354-9

 

Result ID Test Result Name Result LOINC Value
623478 HIV-2 RNA Detect / Quant, P 69354-9