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Test Code PBORB Lyme Disease, Molecular Detection, PCR, Blood

Additional Codes

CNE Order Code: LymePCR,Bld

Reporting Name

Lyme Disease PCR, B

Useful For

Supporting the diagnosis of Lyme disease in conjunction with serologic testing

 

This test should not be used to screen asymptomatic patients.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Ordering Guidance


This assay does not detect Borrelia miyamotoi. If infection with this organism is suspected, order BMIPB / Borrelia miyamotoi Detection, PCR, Blood or BMIYC / Borrelia miyamotoi Detection, PCR, Spinal Fluid.



Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 1 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Reference values apply to all ages.

Day(s) Performed

June through November: Monday through Saturday

December through May: Monday through Friday

CPT Code Information

87476

87798 x 2

87999 (if appropriate for government payers)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PBORB Lyme Disease PCR, B 90892-1

 

Result ID Test Result Name Result LOINC Value
56080 B. burgdorferi PCR, B 94247-4
38290 B. mayonii PCR, B 94248-2
38291 B. garinii/B. afzelii PCR, B 94249-0
38340 Lyme Disease PCR Comment 59464-8

Interpretation

A positive result indicates the presence of DNA from Borrelia burgdorferi, Borrelia mayonii, Borrelia afzelii, or Borrelia garinii, the main agents of Lyme disease.

 

A negative result indicates the absence of detectable target DNA in the specimen. Due to the diagnostic sensitivity limitations of the polymerase chain reaction assay, a negative result does not preclude the presence of the organism or active Lyme disease.

Report Available

1 to 4 days

Reject Due To

Gross hemolysis OK

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Testing Algorithm

For more information see Acute Tick-Borne Disease Testing Algorithm.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.