Sign in →

Test Code PLA2I Phospholipase A2 Receptor, Immunofluorescence, Serum


Ordering Guidance


This test can be used in a complementary fashion with a quantitative enzyme-linked immunosorbent assay to confirm a positive result, especially if it is a low-level titer. Since the immunofluorescence is more sensitive, it can also be used to detect or follow patients with low level antibodies not detected by ELISA.  



Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL 

Collection Instructions: Centrifuge and aliquot serum into a plastic vial within 2 hours of collection.


Useful For

Distinguishing primary from secondary membranous nephropathy in patients with low levels of anti-phospholipase A2 receptor (PLA2R) antibodies

 

Screening for anti-PLA2R antibodies

 

Monitoring patients with membranous nephropathy at very low antibody titers

Method Name

Indirect Immunofluorescence Assay (IFA)

Reporting Name

PLA2R, Immunofluorescence, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  8 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Reference Values

Negative

Interpretation

According to the manufacturer's package insert, the EUROIMMUN Anti-PLA2R indirect immunofluorescence assay was positive in 77.1% of patients with biopsy proven primary membranous nephropathy (pMN). This corresponds well with published literature that approximately 70% of patients with pMN will have anti-phospholipase A2 receptor antibodies.

Day(s) Performed

Monday, Wednesday, Friday

Report Available

3 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86255

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLA2I PLA2R, Immunofluorescence, S 82991-1

 

Result ID Test Result Name Result LOINC Value
PLA2I PLA2R, Immunofluorescence, S 82991-1

Forms

If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.