Test Code PLHBB Plasma Free Hemoglobin, Plasma
Additional Codes
CNE Order Code: Plasma Hgb
Reporting Name
Plasma Free Hemoglobin, PUseful For
Determining whether hemolysis is occurring such as from:
-Transfusion reaction
-Mechanical fragmentation of red blood cells
-Relative comparison to baseline levels in extracorporeal membrane oxygenation and centrifugal ventricular assist device patients to assess pump disruption
Performing Laboratory

Specimen Type
Plasma EDTASpecimen Required
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions:
1. Centrifuge and transfer plasma to a plastic vial within 2 hours of collection.
2. Results could be falsely elevated due to artifactual red blood cell lysis if not centrifuged within 2 hours of collection.
Specimen Minimum Volume
1.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Plasma EDTA | Refrigerated (preferred) | 20 days |
Frozen | 30 days | |
Ambient | 4 days |
Reference Values
TOTAL PLASMA HEMOGLOBIN
≥12 months: 0.0-15.2 mg/dL
Reference values have not been established for patients who are younger than 12 months of age.
OXYHEMOGLOBIN
≥12 months: 0.0-12.4 mg/dL
Reference values have not been established for patients who are younger than 12 months of age.
Day(s) Performed
Monday through Sunday
CPT Code Information
83051
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
PLHBB | Plasma Free Hemoglobin, P | 87433-9 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
31970 | Total Plasma Hemoglobin | 721-1 |
31971 | Oxyhemoglobin | 87437-0 |
Interpretation
An elevation in plasma hemoglobin above the reference range indicates likely intravascular hemolysis due to one of the causes listed in the Useful For section.
Report Available
1 to 3 daysReject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Method Name
Spectrophotometry (SP)
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.