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Test Code POWVB Powassan Virus, RNA, Molecular Detection, PCR, Blood


Ordering Guidance


The Powassan virus polymerase chain reaction result may remain positive for a longer time in urine than in blood, serum, and cerebrospinal fluid (7 days or more).



Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 1 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Useful For

Rapid testing for Powassan virus RNA (lineage 1 and lineage 2) using whole blood specimens

 

An adjunctive test to serology for detection of early Powassan virus infection (ie, first few days after symptom onset)

 

This assay should not be used for screening asymptomatic individuals and should only be used to test patients with signs and symptoms of Powassan virus disease.

Testing Algorithm

For information see the following:

-Meningitis/Encephalitis Panel Algorithm

-Acute Tickborne Disease Testing Algorithm

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Powassan Virus, PCR, Blood

Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Negative

Interpretation

A positive result indicates the presence of Powassan virus RNA and is consistent with early Powassan virus infection.

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
POWVB Powassan Virus, PCR, Blood 34456-4

 

Result ID Test Result Name Result LOINC Value
617488 Lineage 1 34456-4
618144 Lineage 2 (Deer Tick Virus) 34456-4
618148 Powassan Virus PCR Comment 77202-0

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.