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Test Code POWVS Powassan Virus, RNA, Molecular Detection, PCR, Serum


Ordering Guidance


The Powassan virus polymerase chain reaction result may remain positive for a longer time in urine than in blood, serum, and cerebrospinal fluid (7 days or more).



Additional Testing Requirements


This test is designed to be used during the first 7 days after symptom onset and should be ordered in conjunction with POWV / Powassan Virus, IgM, Enzyme-Linked Immunosorbent Assay, Serum.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions:

1. Within 2 hours of collection centrifuge and aliquot serum into a sterile container.

2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.


Useful For

Rapid testing for Powassan virus RNA (lineage 1 and lineage 2) using serum specimens

 

An adjunctive test to serology for detection of early Powassan virus infection (ie, first few days after symptom onset)

 

This assay should not be used for screening asymptomatic individuals and should only be used to test patients with signs and symptoms of Powassan virus disease.

Testing Algorithm

For information see the following:

-Meningitis/Encephalitis Panel Algorithm

-Acute Tickborne Disease Testing Algorithm

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Powassan Virus, PCR, Serum

Specimen Type

Serum

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Gross hemolysis Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Interpretation

A positive result indicates the presence of Powassan virus RNA and is consistent with early Powassan virus infection.

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
POWVS Powassan Virus, PCR, Serum 55144-0

 

Result ID Test Result Name Result LOINC Value
617490 Lineage 1 55144-0
618146 Lineage 2 (Deer Tick Virus) 55144-0
618150 Powassan Virus PCR Comment 77202-0

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.