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HelpTest Code QHV6P Human Herpesvirus-6 A and B DNA Detection and Quantification, PCR, Plasma
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube:
Preferred: Plastic vial
Acceptable: Screw-capped, sterile container
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial.
Useful For
As an adjunct in the rapid diagnosis of human herpesvirus-6 infection using plasma specimens
This test should not be used to screen asymptomatic patients
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Reporting Name
HHV-6 A and B DNA Quant PCR, PSpecimen Type
Plasma EDTASpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 7 days |
| Frozen | 7 days | |
| Ambient | 24 hours |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heparin | Reject |
Reference Values
Undetected
Interpretation
The quantification range of this assay is 500 to 5,000,000 copies/mL (2.70 log to 6.70 log copies/mL)
An "Undetected" test result indicates the absence of human herpesvirus-6 (HHV-6) DNA in plasma.
A test result of "<500 copies/mL (<2.70 log copies/mL)" indicates that HHV-6 DNA is detected in the plasma, but the assay cannot accurately quantify the level of HHV-6 DNA.
A test result of ">5,000,000 copies/mL (>6.70 log copies/mL)" indicates that the HHV-6 DNA level present in plasma is above 5,000,000 copies/mL (6.70 log copies/mL), and the assay cannot accurately quantify the level of HHV-6 DNA. A viral load above 5,000,000 copies/mL should raise suspicion for chromosomally-integrated HHV-6 (ciHHV-6), and additional testing to rule out ciHHV-6 may be needed.
An "Inconclusive" result indicates that the presence or absence of HHV-6 DNA in the plasma specimen could not be determined with certainty after repeat testing in the laboratory, possibly due to inhibition or the presence of an interfering substance. If clinically indicated, submission of a new specimen for testing is recommended.
Day(s) Performed
Monday through Friday
Report Available
4 to 6 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87533
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| QHV6P | HHV-6 A and B DNA Quant PCR, P | 49392-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 622169 | HHV6 A DNA Detect/Quant, P | 49392-4 |
| 622170 | HHV6 B DNA Detect/Quant, P | 49392-4 |
Forms
If not ordering electronically, complete, print, and send an Microbiology Test Request (T244) with the specimen.