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HelpTest Code RPMPM Mycoplasma (Mycoplasmoides) pneumoniae Macrolide (Azithromycin) Resistance Prediction, Molecular Detection, PCR, Varies
Ordering Guidance
This test should only be ordered on specimens that have tested positive for Mycoplasma (Mycoplasmoides) pneumoniae. This assay predicts M pneumoniae macrolide (Azithromycin) resistance only.
For detection of M pneumoniae prior to macrolide resistance testing , order MPRP / Mycoplasma (Mycoplasmoides) pneumoniae with Macrolide Resistance Reflex, Molecular Detection, PCR, Varies.
Necessary Information
Specimen source is required; include the specific anatomic source.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma (Mycoplasmoides) pneumoniae DNA is unlikely.
Submit only 1 of the following specimens:
Specimen Type: Swab
Supplies:
-Culturette (BBL Culture Swab) (T092)
-BD E-swab (T853)
-Nasopharyngeal Swab (Nylon Mini-Tip Swab) (T861)
-Culture Swab-Liquid Stuarts/Single Swab (NP Swab) (T515)
-M4-RT (T605)
Sources: Throat, nasal, or nasopharyngeal
Container/Tube:
Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, universal transport media, or ESwab
Specimen Volume: Swab
Collection Instructions:
1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Specimen Type: Fluid
Sources: Pleural, pericardial, cerebrospinal
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum
Container/Tube: Sterile container
Specimen Volume: 1 mL
Useful For
Predicting macrolide susceptibility in Mycoplasma (Mycoplasmoides) pneumoniae
Method Name
Rapid Polymerase Chain Reaction (PCR) using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)
Reporting Name
M. pneumoniae Macrolide Resist PCRSpecimen Type
VariesSpecimen Minimum Volume
Respiratory: 0.5 mL
Other specimen types: See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reject Due To
| Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal Port-a-Cul tube Anaerobic fluid vials Dry swab (no pledget or sponge) Respiratory fluid specimens placed in viral transport medium (VTM) or placed on a swab and then into VTM (M4-RT, M4, or M5) Body fluid specimens placed in viral transport medium (VTM) or placed on a swab and then in VTM (M4-RT, M4, or M5) |
Reject |
Reference Values
Not Predicted
Interpretation
A macrolide resistance predicted or not predicted result indicates the presence of Mycoplasma (Mycoplasmoides) pneumoniae 23S ribosomal RNA (rRNA) gene and indicates whether one of the 2 most common 23S rRNA gene single nucleotide variations (A2064G and A2063G) associated with high-level macrolide resistance is predicted.
An "unable to assess" result for M pneumoniae macrolide resistance indicates the absence of detectable M pneumoniae 23S rRNA DNA but does not negate the presence of the organism and may occur due to inhibition of the polymerase chain reaction, sequence variability underlying primers or probes, or the presence of M pneumoniae 23S rRNA DNA in quantities less than the limit of detection of the assay.
Day(s) Performed
Monday through Sunday
Report Available
3 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| RPMPM | M. pneumoniae Macrolide Resist PCR | 6483-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRCMP | Specimen source | 31208-2 |
| 619928 | M. pneumoniae Macrolide Resistance | 6483-2 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.