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Test Code RTSC Reptilase Time, Plasma


Specimen Required


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. For complete instruction, see Coagulation Guidelines for Specimen Handling and Processing.

2. Centrifuge, remove plasma, and centrifuge plasma again.

3. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally below -40° C.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Useful For

Evaluation of a prolonged thrombin time (TT): It is mainly used to confirm or exclude the presence of heparin in the specimen or specimen type

 

Evaluating hypofibrinogenemia or dysfibrinogenemia in conjunction with the TT and fibrinogen assay

Method Name

Optical Clot-Based

Reporting Name

Reptilase Time, P

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

14.0-23.9 seconds

Interpretation

As seen in the following table, reptilase time can help distinguish among the various causes of a prolonged thrombin time (TT).

 

Thrombin Time

Reptilase Time

Causes

Remarks

Prolonged

Prolonged

Hypo- or afibrinogenemia

Ascertain by determination of fibrinogen

Prolonged

Prolonged

Dysfibrinogenemia

Ascertain by specific assay

Prolonged

Normal

Heparin or inhibitor of thrombin

Differentiate by human TT and/or heparin assays

Prolonged

Prolonged

Fibrin(ogen) split products (FSP)

Ascertain by FSP or D-dimer assay

 

Day(s) Performed

Monday through Friday

Report Available

1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

85635

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RTSC Reptilase Time, P 6683-7

 

Result ID Test Result Name Result LOINC Value
RTSC Reptilase Time, P 6683-7

Forms

If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.