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HelpTest Code SCOF4 Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2), Influenza Virus Type A and Type B RNA, and Respiratory Syncytial Virus (RSV) Molecular Detection, PCR, Varies
Specimen Required
Preferred:
Specimen Type: Nasopharyngeal swab
Container/Tube: Sterile container with transport media
Specimen Volume: Entire specimen with a minimum of 1.5 mL (maximum 3 mL) of transport media.
Collection Instructions:
1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Swab must be placed into viral transport media (eg, M4-RT, M4 or M5), saline, or phosphate buffered saline (PBS). Media should not contain guanidine thiocyanate (GTC).
Specimen Type: Bronchoalveolar lavage fluid
Container/Tube: Sterile container
Specimen Volume: 0.6 mL
Additional Information: Do not aliquot into viral transport media.
Acceptable:
Specimen Type: Oropharyngeal (throat) swab, nasal mid-turbinate, or nares/nasal swab
Supplies:
-Culturette (BBL Culture Swab) (T092)
-Mid Turbinate (MT) Swab (FLOQSwab/COPAN) (T864)
-Swab, Sterile Polyester (T507)
Container/Tube: Sterile container with transport media
Specimen Volume: Entire specimen with a minimum of 1.5 mL (maximum 3 mL) of transport media.
Preferred: BBL Culture Swab, COPAN Mid-turbinate Swab
Acceptable: Dacron-tipped swab with plastic handle
Collection Instructions: Swab must be placed into viral transport media (eg, M4-RT, M4, or M5), saline, or PBS. Media should not contain guanidine thiocyanate (GTC).
Specimen Type: Bronchial washings, endotracheal aspirate, sputum
Container/Tube: Sterile container
Specimen Volume: 0.6 mL
Additional Information: Do not aliquot into viral transport media.
Useful For
Simultaneous detection and differentiation of SARS-CoV-2 (cause of COVID-19), influenza A virus, influenza B virus, and respiratory syncytial virus in upper or lower respiratory tract specimens from individuals with flu-like illnesses
Testing Algorithm
For information see Coronavirus Disease 2019 (COVID-19), Influenza, and Respiratory Syncytial Virus Testing Algorithm.
Method Name
Multiplex Reverse Transcriptase, Real-Time Polymerase Chain Reaction (RT-qPCR)
Reporting Name
SARS-CoV-2, Flu A/B, and RSV PCR, VSpecimen Type
VariesSpecimen Minimum Volume
Upper respiratory tract swab: See Specimen Required; lower respiratory specimens: 0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Frozen (preferred) | 14 days | |
| Refrigerated | 72 hours |
Reject Due To
| Bloody specimens Calcium alginate-tipped swab Wooden swab Dry swab Glass transport media tubes Transport swab containing gel or charcoal Transport media tubes containing the entire swab Sample tubes containing guanidine isothiocyanate, guanidine thiocyanate, or guanidine hydrochloride |
Reject |
Reference Values
Undetected
Interpretation
A "Detected" (positive) test result indicates that the patient is presumptively infected with the indicated virus. The test does not indicate the stage of infection. Rarely, more than one virus may be detected from the same patient specimen. Laboratory test results should always be considered in the context of clinical observations and epidemiologic data in making a final diagnosis.
An “Undetected" (negative) test result suggests that the patient is not infected with SARS-CoV-2, influenza A virus, influenza B virus, or respiratory syncytial virus (RSV).
An "Inconclusive" result indicates that the presence or absence of SARS-CoV-2, influenza A virus, influenza B virus, and RSV in the specimen could not be determined with certainty after repeat testing in the laboratory, possibly due to real-time, reverse transcription polymerase chain reaction inhibition. Submission of a new specimen for testing is recommended.
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 2 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87637
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SCOF4 | SARS-CoV-2, Flu A/B, and RSV PCR, V | 95941-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SCOS4 | SARS-CoV-2 & Flu A/B, Source | 31208-2 |
| 622032 | Influenza A virus PCR | 92142-9 |
| 622033 | Influenza B virus PCR | 92141-1 |
| 622034 | SARS CoV-2 RNA, PCR | 94500-6 |
| 622035 | Respiratory Syncytial Virus, PCR | 92131-2 |