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HelpTest Code TCP T-Cell Subsets, Naive, Memory, and Activated, Blood
Reporting Name
T Cell Phenotyping, AdvancedUseful For
Determining the presence of naive, memory, and activated T cells in various clinical contexts including autoimmune diseases, immunodeficiency states, T-cell recovery post-hematopoietic stem cell transplant, DiGeorge syndrome, and as a measure for T-cell immune competence
Naive T-cells results can be used as a surrogate marker for thymic-derived T-cell reconstitution, when used in conjunction with assessment of T-cell receptor excision circles (TRECS / T-Cell Receptor Excision Circles Analysis, Blood)
Assessing a patient's relative risk for infections
Evaluating patients with cellular or combined primary immunodeficiencies
Evaluating T-cell reconstitution after hematopoietic stem cell transplant, chemotherapy, biological therapy, immunosuppression, or immunomodulator therapy
Evaluating patients with autoimmune diseases
Evaluating patients who are HIV-positive for naive and memory subsets
Evaluating T-cell immune competence (presence of memory and activated T cells) in patients with recurrent infections
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAOrdering Guidance
This assay provides quantitative information on various T-cell subsets in blood; it does not provide any information on the antigen-specific or otherwise functional state of the T cells.
To assess the overall functional state of T cells, order either LPMGF / Lymphocyte Proliferation to Mitogens, Blood or LPAGF / Lymphocyte Proliferation to Antigens, Blood (using Candida and tetanus antigens).
To assess cytomegalovirus (CMV)-specific immune competence, order CMVC8 / Cytomegalovirus (CMV) CD8 T-Cell Immune Competence, Quantitative Assessment by Flow Cytometry, Blood.
Shipping Instructions
Testing performed Monday through Friday. Specimens not received by 4 p.m. Central time on Fridays may be canceled.
Specimens arriving on the weekend and observed holidays may be canceled.
Collect and package specimen as close to shipping time as possible.
It is recommended that specimens arrive within 24 hours of collection.
Necessary Information
Ordering healthcare professional's name and phone number are required.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Additional Information: For serial monitoring, it is recommended that specimens are collected at the same time of day.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
Reference Values
The appropriate age-related reference values will be provided on the report.
Day(s) Performed
Monday through Friday
CPT Code Information
86356 x 7
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TCP | T Cell Phenotyping, Advanced | 96493-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 609282 | CD4 (T Cells) | 24467-3 |
| 609283 | CD8 (T Cells) | 14135-8 |
| 29151 | %CD4+CD45RA+ naive T cells | 89360-2 |
| 29152 | %CD4+CD62L+CD27+ naive T cells | 89340-4 |
| 29153 | %CD8+CD45RA+ naive T cells | 82744-4 |
| 29154 | %CD8+CD62L+CD27+naive T cells | 89339-6 |
| 29155 | %CD4+CD45RO+ memory T cells | 89362-8 |
| 29156 | %CD4+CD62L+CD27+CD45RO+ (Tcm) | 89338-8 |
| 29157 | %CD4+CD62L-CD27-CD45RO+ (Tem) | 89337-0 |
| 29158 | %CD8+CD45RO+ memory T cells | 89336-2 |
| 29159 | %CD8+CD62L+CD27+CD45RO+ (Tcm) | 89335-4 |
| 29160 | %CD8+CD62L-CD27-CD45RO+ (Tem) | 89334-7 |
| 29161 | %Activated CD4 T cells (4+CD25+) | 89431-1 |
| 29162 | %CD4+HLA DR+CD28+ T cells | 89333-9 |
| 29163 | %CD8+HLA DR+CD28+ T cells | 89332-1 |
| 29164 | CD4+CD45RA+ naive T cells | 26759-1 |
| 29165 | CD4+CD62L+CD27+ naive T cells | 89331-3 |
| 29166 | CD8+CD45RA+ naive T cells | 82743-6 |
| 29167 | CD8+CD62L+CD27+naive T cells | 89330-5 |
| 29168 | CD4+CD45RO+ memory T cells | 85792-0 |
| 29169 | CD4+CD62L+CD27+CD45RO+ (Tcm) | 89329-7 |
| 29170 | CD4+CD62L-CD27-CD45RO+ (Tem) | 89328-9 |
| 29171 | CD8+CD45RO+ memory T cells | 85790-4 |
| 29172 | CD8+CD62L+CD27+CD45RO+ (Tcm) | 96492-4 |
| 29173 | CD8+CD62L-CD27- CD45RO+ (Tem) | 89327-1 |
| 29174 | Activated CD4 T cells (4+CD25+) | 26982-9 |
| 29175 | CD4+HLA DR+CD28+ T cells | 89326-3 |
| 29176 | CD8+HLA DR+CD28+ T cells | 89325-5 |
| 29178 | Interpretation | 69052-9 |
Interpretation
Absence or reduction of naive T cells with or without T-cell lymphopenia indicates absent or impaired T-cell reconstitution or thymic output. Reduction in activated T cells can also indicate a reduced T-cell immune competent state.
Increases in naive T cells with corresponding decreases in the memory T-cell compartment indicates a failure of further differentiation and effector function or selective loss of memory T cells and an increased risk for infection.
Report Available
3 to 4 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
Method Name
Flow Cytometry